PGE₂ is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells.

ABSTRACT: Intestinal epithelial cells (IECs) play an indispensable role in maintaining body fluid balance partly through their ability to regulate anion/fluid secretion. Yet in various inflammatory gastrointestinal diseases, over-secretion of anions results in symptoms such as severe diarrhoea. Endogenous mediators, such as vasoactive intestinal peptide or prostaglandin E₂ (PGE₂), regulate intestinal anion/fluid secretion, but their direct effect on purified human IECs has never been described in detail. Based on a previously described intestinal organoid swelling model, we established a 3D-scanner-assisted quantification method to evaluate the anion/fluid secretory response of cultured human IECs. Among various endogenous secretagogues, we found that PGE₂ had the lo

SUBMITTER: Fujii S 

PROVIDER: S-EPMC5101536 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature