Identification and characterization of microRNAs expressed in the African malaria vector Anopheles funestus life stages using high throughput sequencing.

ABSTRACT:

Background

Over the past several years, thousands of microRNAs (miRNAs) have been identified in the genomes of various insects through cloning and sequencing or even by computational prediction. However, the number of miRNAs identified in anopheline species is low and little is known about their role. The mosquito Anopheles funestus is one of the dominant malaria vectors in Africa, which infects and kills millions of people every year. Therefore, small RNA molecules isolated from the four life stages (eggs, larvae, pupae and unfed adult females) of An. funestus were sequenced using next generation sequencing technology.

Results

High throughput sequencing of four replicates in combination with computational analysis identified 107 mature miRNA sequences expressed in the An. f

SUBMITTER: Allam M 

PROVIDER: S-EPMC5101901 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature