Systematic Characterization of Dynamic Parameters of Intracellular Calcium Signals.

ABSTRACT: Dynamic processes, such as intracellular calcium signaling, are hallmark of cellular biology. As real-time imaging modalities become widespread, a need for analytical tools to reliably characterize time-series data without prior knowledge of the nature of the recordings becomes more pressing. The goal of this study is to develop a signal-processing algorithm for MATLAB that autonomously computes the parameters characterizing prominent single transient responses (TR) and/or multi-peaks responses (MPR). The algorithm corrects for signal contamination and decomposes experimental recordings into contributions from drift, TRs, and MPRs. It subsequently provides numerical estimates for the following parameters: time of onset after stimulus application, activation time (time for signal to increas

SUBMITTER: Mackay L 

PROVIDER: S-EPMC5102910 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature