A new assay for the simultaneous identification and differentiation of Klebsiella oxytoca strains.

ABSTRACT: Klebsiella oxytoca is the second most frequently identified species of Klebsiella isolated from hospitalized patients. Klebsiella spp. is difficult to identify using conventional methods and is often misclassified in clinical microbiology laboratories. K. oxytoca is responsible for an increasing number of multi-resistant infections in hospitals because of insufficient detection and identification. In this study, we propose a new simple method called pehX-LM PCR/XbaI, which simultaneously indicates K. oxytoca species and genotype by the fingerprint pattern. The pehX-LM PCR/XbaI is a combination of the following two methods: species-specific amplification of pehX gene and non-specific amplification of short restriction fragments by the LM PCR method. The specificity and the discrimination po

SUBMITTER: Stojowska-Swedrzynska K 

PROVIDER: S-EPMC5102950 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature