Amplicon-Based RNA Interference Targeting V2 Gene of Cotton Leaf Curl Kokhran Virus-Burewala Strain Can Provide Resistance in Transgenic Cotton Plants.

ABSTRACT: The conserved coat or V2 gene of begomoviruses is responsible for viral movement in the plant cells. RNAi technology was used to silence V2 gene for resistance against these viruses in transgenic plants. The transformation of the RNAi-based gene construct targeting V2 gene of CLCuKoV-Bur, cloned under 35S promoter, was done in two elite cotton varieties MNH-786 and VH-289 using shoot apex cut method of gene transformation. The transformation efficiency was found to be 3.75 and 2.88 % in MNH-786 and VH-289, respectively. Confirmation of successful transformation was done through PCR in T ₀, T ₁, and T ₂ generations using gene-specific primers. Transgenic cotton plants were categorized on the basis of the virus disease index in T ₁ generation. Copy

SUBMITTER: Yasmeen A 

PROVIDER: S-EPMC5102983 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature