Challenges in Predicting Protein-Protein Interactions from Measurements of Molecular Diffusivity.

ABSTRACT: Dynamic light scattering can be used to measure the diffusivity of a protein within a formulation. The dependence of molecular diffusivity on protein concentration (traditionally expressed in terms of the interaction parameter k_D) is often used to infer whether protein-protein interactions are repulsive or attractive, resulting in solutions that are colloidally stable or unstable, respectively. However, a number of factors unrelated to intermolecular forces can also impact protein diffusion, complicating this interpretation. Here, we investigate the influence of multicomponent diffusion in a ternary protein-salt-water system on protein diffusion and k_D in the context of Nernst-Planck theory. This analysis demonstrates that large changes in protein diffusivity with pro

SUBMITTER: Sorret LL 

PROVIDER: S-EPMC5103004 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature