Basis of Protein Stabilization by K Glutamate: Unfavorable Interactions with Carbon, Oxygen Groups.

ABSTRACT: Potassium glutamate (KGlu) is the primary Escherichia coli cytoplasmic salt. After sudden osmotic upshift, cytoplasmic KGlu concentration increases, initially because of water efflux and subsequently by K⁺ transport and Glu^- synthesis, allowing water uptake and resumption of growth at high osmolality. In vitro, KGlu ranks with Hofmeister salts KF and K₂SO₄ in driving protein folding and assembly. Replacement of KCl by KGlu stabilizes protein-nucleic acid complexes. To interpret and predict KGlu effects on protein processes, preferential interactions of KGlu with 15 model compounds displaying six protein functional groups-sp³ (aliphatic) C; sp² (aromatic, amide, carboxylate) C; amide and anionic (carboxylate) O; and amide and

SUBMITTER: Cheng X 

PROVIDER: S-EPMC5103011 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature