Oriented Membrane Protein Reconstitution into Tethered Lipid Membranes for AFM Force Spectroscopy.

ABSTRACT: Membrane proteins act as a central interface between the extracellular environment and the intracellular response and as such represent one of the most important classes of drug targets. The characterization of the molecular properties of integral membrane proteins, such as topology and interdomain interaction, is key to a fundamental understanding of their function. Atomic force microscopy (AFM) and force spectroscopy have the intrinsic capabilities of investigating these properties in a near-native setting. However, atomic force spectroscopy of membrane proteins is traditionally carried out in a crystalline setup. Alternatively, model membrane systems, such as tethered bilayer membranes, have been developed for surface-dependent techniques. While these setups can provide a more native en

SUBMITTER: Bronder AM 

PROVIDER: S-EPMC5103026 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature