The Redox Cofactor F₄₂₀ Protects Mycobacteria from Diverse Antimicrobial Compounds and Mediates a Reductive Detoxification System.

ABSTRACT: A defining feature of mycobacterial redox metabolism is the use of an unusual deazaflavin cofactor, F₄₂₀ This cofactor enhances the persistence of environmental and pathogenic mycobacteria, including after antimicrobial treatment, although the molecular basis for this remains to be understood. In this work, we explored our hypothesis that F₄₂₀ enhances persistence by serving as a cofactor in antimicrobial-detoxifying enzymes. To test this, we performed a series of phenotypic, biochemical, and analytical chemistry studies in relation to the model soil bacterium Mycobacterium smegmatis Mutant strains unable to synthesize or reduce F₄₂₀ were found to be more susceptible to a wide range of antibiotic and xenobiotic compounds. Compounds from three classes

SUBMITTER: Jirapanjawat T 

PROVIDER: S-EPMC5103081 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature