Generation of a Stable Plasmid for In Vitro and In Vivo Studies of Staphylococcus Species.

ABSTRACT: A major shortcoming to plasmid-based genetic tools is the necessity of using antibiotics to ensure plasmid maintenance. While selectable markers are very powerful, their use is not always practical, such as during in vivo models of bacterial infection. During previous studies, it was noted that the uncharacterized LAC-p01 plasmid in Staphylococcus aureus USA300 isolates was stable in the absence of a known selection and therefore could serve as a platform for new genetic tools for Staphylococcus species. LAC-p01 was genetically manipulated into an Escherichia coli-S. aureus shuttle vector that remained stable for at least 100 generations without antibiotic selection. The double- and single-stranded (dso and sso) origins were identified and f

SUBMITTER: Krute CN 

PROVIDER: S-EPMC5103085 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature