Display of whole proteins on inner and outer surfaces of grapevine fanleaf virus-like particles.

ABSTRACT: Virus-like particles (VLPs) derived from nonenveloped viruses result from the self-assembly of capsid proteins (CPs). They generally show similar structural features to viral particles but are noninfectious and their inner cavity and outer surface can potentially be adapted to serve as nanocarriers of great biotechnological interest. While a VLP outer surface is generally amenable to chemical or genetic modifications, encaging a cargo within particles can be more complex and is often limited to small molecules or peptides. Examples where both inner cavity and outer surface have been used to simultaneously encapsulate and expose entire proteins remain scarce. Here, we describe the production of spherical VLPs exposing fluorescent proteins at either their outer surface or inner cavity as a r

SUBMITTER: Belval L 

PROVIDER: S-EPMC5103221 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature