High Intratumoral Stromal Content Defines Reactive Breast Cancer as a Low-risk Breast Cancer Subtype.

ABSTRACT:

Purpose

The current study evaluated associative effects of breast cancer cells with the tumor microenvironment and its influence on tumor behavior.

Experimental design

Formalin-fixed, paraffin-embedded tissue and matched protein lysates were evaluated from two independent breast cancer patient datasets (TCGA and MD Anderson). Reverse-phase protein arrays (RPPA) were utilized to create a proteomics signature to define breast tumor subtypes. Expression patterns of cell lines and normal breast tissues were utilized to determine markers that were differentially expressed in stroma and cancer cells. Protein localization and stromal contents were evaluated for matched cases by imaging.

Results

A subtype of breast cancers designated "Reactive," previously identified by RPPA

SUBMITTER: Dennison JB 

PROVIDER: S-EPMC5103285 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature