Suppressed translation and ULK1 degradation as potential mechanisms of autophagy limitation under prolonged starvation.

ABSTRACT: Macroautophagy/autophagy is a well-organized process of intracellular degradation, which is rapidly activated under starvation conditions. Recent data demonstrate a transcriptional upregulation of several autophagy genes as a mechanism that controls autophagy in response to starvation. Here we report that despite the significant upregulation of mRNA of the essential autophagy initiation gene ULK1, its protein level is rapidly reduced under starvation. Although both autophagic and proteasomal systems contribute to the degradation of ULK1, under prolonged nitrogen deprivation, its level was still reduced in ATG7 knockout cells, and only initially stabilized in cells treated with the lysosomal or proteasomal inhibitors. We demonstrate that under starvation, protein translation is rapidly dimi

SUBMITTER: Allavena G 

PROVIDER: S-EPMC5103336 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature