Detection of the first G6P[14] human rotavirus strain in an infant with diarrhoea in Ghana.

ABSTRACT:

Background

Rotaviruses with G6P[14] specificity are mostly isolated in cattle and have been established as a rare cause of gastroenteritis in humans. This study reports the first detection of G6P[14] rotavirus strain in Ghana from the stool of an infant during a hospital-based rotavirus surveillance study in 2010.

Methods

Viral RNA was extracted and rotavirus VP7 and VP4 genes amplified by one step RT-PCR using gene-specific primers. The DNA was purified, sequenced and genotypes determined using BLAST and RotaC v2.0. Phylogenetic tree was constructed using maximum likelihood method in MEGA v6.06 software and statistically supported by bootstrapping with 1000 replicates. Phylogenetic distances were calculated using the Kimura-2 parameter model.

Results

The study strain

SUBMITTER: Damanka S 

PROVIDER: S-EPMC5103419 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature