LSCplus: a fast solution for improving long read accuracy by short read alignment.

ABSTRACT:

Background

The single molecule, real time (SMRT) sequencing technology of Pacific Biosciences enables the acquisition of transcripts from end to end due to its ability to produce extraordinarily long reads (>10 kb). This new method of transcriptome sequencing has been applied to several projects on humans and model organisms. However, the raw data from SMRT sequencing are of relatively low quality, with a random error rate of approximately 15 %, for which error correction using next-generation sequencing (NGS) short reads is typically necessary. Few tools have been designed that apply a hybrid sequencing approach that combines NGS and SMRT data, and the most popular existing tool for error correction, LSC, has computing resource requirements that are too intensive for most laborato

SUBMITTER: Hu R 

PROVIDER: S-EPMC5103424 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature