In vivo inhibition of miR-155 significantly alters post-stroke inflammatory response.

ABSTRACT:

Background

MicroRNA miR-155 is implicated in modulation of the inflammatory processes in various pathological conditions. In our previous studies, we demonstrated that in vivo inhibition of miR-155 promotes functional recovery after mouse experimental stroke. In the present study, we explored if this beneficial effect is associated with miR-155 inhibition-induced alterations in post-stroke inflammatory response.

Methods

Intravenous injections of a specific miR-155 inhibitor were initiated at 48 h after mouse distal middle cerebral artery occlusion (dMCAO). Temporal changes in the expression of cytokines and key molecules associated with cytokine signaling were assessed at 7, 14, and 21 days after dMCAO, using mouse cytokine gene and protein arrays and Western blot analyses.

SUBMITTER: Pena-Philippides JC 

PROVIDER: S-EPMC5103429 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature