Short tandem repeats, segmental duplications, gene deletion, and genomic instability in a rapidly diversified immune gene family.

ABSTRACT:

Background

Genomic regions with repetitive sequences are considered unstable and prone to swift DNA diversification processes. A highly diverse immune gene family of the sea urchin (Strongylocentrotus purpuratus), called Sp185/333, is composed of clustered genes with similar sequence as well as several types of repeats ranging in size from short tandem repeats (STRs) to large segmental duplications. This repetitive structure may have been the basis for the incorrect assembly of this gene family in the sea urchin genome sequence. Consequently, we have resolved the structure of the family and profiled the members by sequencing selected BAC clones using Illumina and PacBio approaches.

Results

BAC insert assemblies identified 15 predicted genes that are organized into three clus

SUBMITTER: Oren M 

PROVIDER: S-EPMC5103432 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature