The bronchial epithelial cell bacterial microbiome and host response in patients infected with human immunodeficiency virus.

ABSTRACT:

Background

Chronic Obstructive Pulmonary Disease (COPD) is an important comorbidity in patients living with human immunodeficiency virus (HIV). Previous bacterial microbiome studies have shown increased abundance of specific bacterium, like Tropheryma whipplei, and no overall community differences. However, the host response to the lung microbiome is unknown in patients infected with HIV.

Methods

Two bronchial brush samples were obtained from 21 HIV-infected patients. One brush was used for bacterial microbiome analysis using the Illumina MiSeq^TM platform, while the other was used to evaluate gene expression patterns of the host using the Affymetrix Human Gene ST 2.0 array. Weighted gene co-expression network analysis was used to determine the relationship between

SUBMITTER: Sze MA 

PROVIDER: S-EPMC5103452 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature