Bacterial communities associated with apical periodontitis and dental implant failure.

ABSTRACT:

Background

Previously, we demonstrated that bacteria reside in apparently healed alveolar bone, using culture and Sanger sequencing techniques. Bacteria in apparently healed alveolar bone may have a role in peri-implantitis and dental implant failure.

Objective

To compare bacterial communities associated with apical periodontitis, those colonising a failed implant and alveolar bone with reference biofilm samples from healthy teeth.

Methods and results

The study consisted of 196 samples collected from 40 patients undergoing routine dental implant insertion or rehabilitation. The bacterial 16S ribosomal DNA sequences were amplified. Samples yielding sufficient polymerase chain reaction product for further molecular analyses were subjected to terminal restriction fragmen

SUBMITTER: Dingsdag S 

PROVIDER: S-EPMC5103668 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature