Cloning, expression and characterization of a cold-adapted endo-1, 4-β-glucanase from Citrobacter farmeri A1, a symbiotic bacterium of Reticulitermes labralis.

ABSTRACT:

Background

Many biotechnological and industrial applications can benefit from cold-adapted EglCs through increased efficiency of catalytic processes at low temperature. In our previous study, Citrobacter farmeri A1 which was isolated from a wood-inhabiting termite Reticulitermes labralis could secrete a cold-adapted EglC. However, its EglC was difficult to purify for enzymatic properties detection because of its low activity (0.8 U/ml). The objective of the present study was to clone and express the C. farmeri EglC gene in Escherichia coli to improve production level and determine the enzymatic properties of the recombinant enzyme.

Methods

The EglC gene was cloned from C. farmeri A1 by thermal asymmetric interlaced PCR. EglC w

SUBMITTER: Bai X 

PROVIDER: S-EPMC5103814 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature