Iron overload induced death of osteoblasts in vitro: involvement of the mitochondrial apoptotic pathway.

ABSTRACT:

Background

Iron overload is recognized as a new pathogenfor osteoporosis. Various studies demonstrated that iron overload could induce apoptosis in osteoblasts and osteoporosis in vivo. However, the exact molecular mechanisms involved in the iron overload-mediated induction of apoptosis in osteoblasts has not been explored.

Purpose

In this study, we attempted to determine whether the mitochondrial apoptotic pathway is involved in iron-induced osteoblastic cell death and to investigate the beneficial effect of N-acetyl-cysteine (NAC) in iron-induced cytotoxicity.

Methods

The MC3T3-E1 osteoblastic cell line was treated with various concentrations of ferric ion in the absence or presence of NAC, and intracellular iron, cell viability, reactive oxygen species, functionand

SUBMITTER: Tian Q 

PROVIDER: S-EPMC5103817 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature