Crystal Structure of the Neuropilin-1 MAM Domain: Completing the Neuropilin-1 Ectodomain Picture.

ABSTRACT: Neuropilins (NRPs) are single-pass transmembrane receptors involved in several signaling pathways that regulate key physiological processes such as vascular morphogenesis and axon guidance. The MAM domain of NRP, which has previously been implicated in receptor multimerization, was the only portion of the ectopic domain of the NRPs for which the structure, until now, has been elusive. Using site-directed mutagenesis in the linker region preceding the MAM domain we generated a protein construct amenable to crystallization. Here we present the crystal structure of the MAM domain of human NRP1 at 2.24 Å resolution. The protein exhibits a jellyroll topology, with Ca²⁺ ions bound at the inter-strand space enhancing the thermostability of the domain. We show that the MAM domain of NRP

SUBMITTER: Yelland T 

PROVIDER: S-EPMC5104691 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature