Expression of heat-shock protein 72 mRNA in relation to heart rate variability of Sahiwal and Karan-Fries in different temperature-humidity indices.

ABSTRACT:

Aim

To investigate the effect of temperature-humidity index (THI) on the expression pattern of heat-shock protein 72 (HSP72) mRNA of Sahiwal and Karan-Fries (KF) cattle in different THIs.

Materials and methods

Five different periods of a year were selected based on combinations of T_max/T_min, viz., P1: <20°C/<10°C; P2: >20°C/<10°C, P3: <30°C/<15°C; P4: >35°C/<20°C, and P5: >35°C/>20°C. The THI was calculated from the records of temperature and relative humidity in different periods. Heart rate variability (HRV) was calculated from electrocardiogram records in different periods. HSP72 mRNA expression was estimated by reverse transcription polymerase chain reaction.

Results

The THI recorded during P1, P2, P3, P4, and P5 were 55.5, 60.3, 70.1, 74.5,

SUBMITTER: Mayengbam P 

PROVIDER: S-EPMC5104711 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature