Polyadenylated tail length variation pattern in ultra-rapid vitrified bovine oocytes.

ABSTRACT:

Aim

Thecurrent study aims at investigating the polyadenylated (poly[A]) tail length of morphologically high and low competent oocytes at different developmental stages. Furthermore, effect of ultra-rapid vitrification on the poly(A) tail length was studied.

Materials and methods

Fresh bovine cumulus oocyte complexes from abattoir originated ovaries were graded based on morphological characters and matured in vitro. Cryopreservation was done by ultra-rapid vitrification method. mRNA was isolated from different categories of oocyte and subjected to ligation-mediated poly(A) test followed by polymerase chain reaction for determining the poly(A) tail length of β actin, gap junction protein alpha 1 (GJA1), poly(A) polymerase alpha (PAPOLA), and heat shock 70 kDa protein (H

SUBMITTER: Dutta DJ 

PROVIDER: S-EPMC5104714 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature