Marker-free PLRV resistant potato mediated by Cre-loxP excision and RNAi.

ABSTRACT: An inverted repeat construct corresponding to a segment of the potato leaf roll virus coat protein gene was created under control of a constitutive promoter and transferred into a transformation vector with a heat inducible Cre-loxP system to excise the nptII antibiotic resistance marker gene. Fifty-eight transgenic events were evaluated for resistance to PLRV by greenhouse inoculations, which lead to the identification of 7 highly resistant events, of which 4 were extremely resistant. This resistance was also highly effective against accumulation in subsequent tuber generations from inoculated plants, which has not been reported before. Northern blot analysis showed correlation of PLRV specific siRNA accumulation with the level of PLRV resistance. Heat mediated excision of the nptII antib

SUBMITTER: Orbegozo J 

PROVIDER: S-EPMC5104775 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature