A New Splice Variant of Large Conductance Ca2+-activated K+ (BK) Channel α Subunit Alters Human Chondrocyte Function.

ABSTRACT: Large conductance Ca²⁺-activated K⁺ (BK) channels play essential roles in both excitable and non-excitable cells. For example, in chondrocytes, agonist-induced Ca²⁺ release from intracellular store activates BK channels, and this hyperpolarizes these cells, augments Ca²⁺ entry, and forms a positive feed-back mechanism for Ca²⁺ signaling and stimulation-secretion coupling. In the present study, functional roles of a newly identified splice variant in the BK channel α subunit (BKαΔe2) were examined in a human chondrocyte cell line, OUMS-27, and in a HEK293 expression system. Although BKαΔe2 lacks exon2, which codes the intracellular S0-S1 linker (Glu-127-Leu-180), significant expression was detected in several tissues from humans and mic

SUBMITTER: Suzuki Y 

PROVIDER: S-EPMC5104946 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature