Hybrid mass spectrometry approaches in glycoprotein analysis and their usage in scoring biosimilarity.

ABSTRACT: Many biopharmaceutical products exhibit extensive structural micro-heterogeneity due to an array of co-occurring post-translational modifications. These modifications often effect the functionality of the product and therefore need to be characterized in detail. Here, we present an integrative approach, combining two advanced mass spectrometry-based methods, high-resolution native mass spectrometry and middle-down proteomics, to analyse this micro-heterogeneity. Taking human erythropoietin and the human plasma properdin as model systems, we demonstrate that this strategy bridges the gap between peptide- and protein-based mass spectrometry platforms, providing the most complete profiling of glycoproteins. Integration of the two methods enabled the discovery of three undescribed C-glycosylat

SUBMITTER: Yang Y 

PROVIDER: S-EPMC5105167 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature