Genetic tools for advancement of Synechococcus sp. PCC 7002 as a cyanobacterial chassis.

ABSTRACT:

Background

Successful implementation of modified cyanobacteria as hosts for industrial applications requires the development of a cyanobacterial chassis. The cyanobacterium Synechococcus sp. PCC 7002 embodies key attributes for an industrial host, including a fast growth rate and high salt, light, and temperature tolerances. This study addresses key limitations in the advancement of Synechococcus sp. PCC 7002 as an industrial chassis.

Results

Tools for genome integration were developed and characterized, including several putative neutral sites for genome integration. The minimum homology arm length for genome integration in Synechococcus sp. PCC 7002 was determined to be approximately 250 bp. Three fluorescent protein reporters (hGFP, Ypet, and mOrange) were characterized f

SUBMITTER: Ruffing AM 

PROVIDER: S-EPMC5105302 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature