SOX2, OCT3/4 and NANOG expression and cellular plasticity in rare human somatic cells requires CD73.

ABSTRACT: Endogenous Plastic Somatic (ePS) cells isolated from adult human tissues exhibit extensive lineage plasticity in vitro and in vivo. Here we visualize these rare ePS cells in a latent state, i.e. lacking SOX2, OCT3/4 and NANOG (SON) expression, in non-diseased breast specimens through immunohistochemical analysis of previously identified ePS-specific biomarkers (CD73⁺, EpCAM⁺ and CD90^-). We also report a novel mechanism by which these latent ePS cells acquire SON expression and plasticity in vitro. Four extracellular factors are necessary for the acquisition of SON expression and lineage plasticity in ePS cells: adenosine (which is produced by the 5' ecto-nucleotidase CD73 and activates in turn the PKA-dependent IL6/STAT3 pathway through the adenosine recept

SUBMITTER: Pan D 

PROVIDER: S-EPMC5107210 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature