Ontogeny of human IgE-expressing B cells and plasma cells.

ABSTRACT:

Background

IgE-expressing (IgE⁺ ) plasma cells (PCs) provide a continuous source of allergen-specific IgE that is central to allergic responses. The extreme sparsity of IgE⁺ cells in vivo has confined their study almost entirely to mouse models.

Objective

To characterize the development pathway of human IgE⁺ PCs and to determine the ontogeny of human IgE⁺ PCs.

Methods

To generate human IgE⁺ cells, we cultured tonsil B cells with IL-4 and anti-CD40. Using FACS and RT-PCR, we examined the phenotype of generated IgE⁺ cells, the capacity of tonsil B-cell subsets to generate IgE⁺ PCs and the class switching pathways involved.

Results

We have identified three phenotypic stages of IgE^+</

SUBMITTER: Ramadani F 

PROVIDER: S-EPMC5107308 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature