Proteome analysis of mast cell releasates reveals a role for chymase in the regulation of coagulation factor XIIIA levels via proteolytic degradation.

ABSTRACT:

Background

Mast cells are significantly involved in IgE-mediated allergic reactions; however, their roles in health and disease are incompletely understood.

Objective

We aimed to define the proteome contained in mast cell releasates on activation to better understand the factors secreted by mast cells that are relevant to the contribution of mast cells in diseases.

Methods

Bone marrow-derived cultured mast cells (BMCMCs) and peritoneal cell-derived mast cells were used as "surrogates" for mucosal and connective tissue mast cells, respectively, and their releasate proteomes were analyzed by mass spectrometry.

Results

Our studies showed that BMCMCs and peritoneal cell-derived mast cells produced substantially different releasates following IgE-mediated activation

SUBMITTER: Shubin NJ 

PROVIDER: S-EPMC5107356 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature