Proteomic characterization of neuromelanin granules isolated from human substantia nigra by laser-microdissection.

ABSTRACT: Neuromelanin is a complex polymer pigment found primarily in the dopaminergic neurons of human substantia nigra. Neuromelanin pigment is stored in granules including a protein matrix and lipid droplets. Neuromelanin granules are yet only partially characterised regarding their structure and function. To clarify the exact function of neuromelanin granules in humans, their enrichment and in-depth characterization from human substantia nigra is necessary. Previously published global proteome studies of neuromelanin granules in human substantia nigra required high tissue amounts. Due to the limited availability of human brain tissue we established a new method based on laser microdissection combined with mass spectrometry for the isolation and analysis of neuromelanin granules. With this metho

SUBMITTER: Plum S 

PROVIDER: S-EPMC5107900 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature