5-azacytidine affects TET2 and histone transcription and reshapes morphology of human skin fibroblasts.

ABSTRACT: Phenotype definition is controlled by epigenetic regulations that allow cells to acquire their differentiated state. The process is reversible and attractive for therapeutic intervention and for the reactivation of hypermethylated pluripotency genes that facilitate transition to a higher plasticity state. We report the results obtained in human fibroblasts exposed to the epigenetic modifier 5-azacytidine (5-aza-CR), which increases adult cell plasticity and facilitates phenotype change. Although many aspects controlling its demethylating action have been widely investigated, the mechanisms underlying 5-aza-CR effects on cell plasticity are still poorly understood. Our experiments confirm decreased global methylation, but also demonstrate an increase of both Formylcytosine (5fC) and 5-Carbo

SUBMITTER: Manzoni EF 

PROVIDER: S-EPMC5107985 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature