3D Mass Spectrometry Imaging Reveals a Very Heterogeneous Drug Distribution in Tumors.

ABSTRACT: Mass Spectrometry Imaging (MSI) is a widespread technique used to qualitatively describe in two dimensions the distribution of endogenous or exogenous compounds within tissue sections. Absolute quantification of drugs using MSI is a recent challenge that just in the last years has started to be addressed. Starting from a two dimensional MSI protocol, we developed a three-dimensional pipeline to study drug penetration in tumors and to develop a new drug quantification method by MALDI MSI. Paclitaxel distribution and concentration in different tumors were measured in a 3D model of Malignant Pleural Mesothelioma (MPM), which is known to be a very heterogeneous neoplasm, highly resistant to different drugs. The 3D computational reconstruction allows an accurate description of tumor PTX penetra

SUBMITTER: Giordano S 

PROVIDER: S-EPMC5107992 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature