Evaluating Digital PCR for the Quantification of Human Genomic DNA: Accessible Amplifiable Targets.

ABSTRACT: Polymerase chain reaction (PCR) multiplexed assays perform best when the input quantity of template DNA is controlled to within about a factor of √2. To help ensure that PCR assays yield consistent results over time and place, results from methods used to determine DNA quantity need to be metrologically traceable to a common reference. Many DNA quantitation systems can be accurately calibrated with solutions of DNA in aqueous buffer. Since they do not require external calibration, end-point limiting dilution technologies, collectively termed "digital PCR (dPCR)", have been proposed as suitable for value assigning such DNA calibrants. The performance characteristics of several commercially available dPCR systems have recently been documented using plasmid, viral, or fragmented genomic DNA;

SUBMITTER: Kline MC 

PROVIDER: S-EPMC5108050 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature