Early Cellular Response to Radiation in Human Vocal Fold Fibroblasts.

ABSTRACT:

Objectives

Radiation therapy is a common treatment strategy for laryngeal carcinoma. However, radiation is not without adverse side effects, especially toward healthy vocal fold tissue, which can lead to long-term impairments in vocal function. The objective of this preliminary study was to investigate early responses of healthy human vocal fold fibroblasts (VFF) to radiation.

Methods

VFF were exposed to a single or fractionated dose radiation scheme. Nonradiated VFF served as controls. Morphology of radiated and control VFF was subjectively examined. Quantitative polymerase chain reaction was used to evaluate the effect of radiation on extracellular matrix and inflammatory-related genes. VFF viability was investigated using a LIVE/DEAD and clonogenic assay.

Results

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SUBMITTER: Erickson-DiRenzo E 

PROVIDER: S-EPMC5108093 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature