A diagnostic one-step real-time reverse transcription polymerase chain reaction method for accurate detection of influenza virus type A.

ABSTRACT:

Introduction

Influenza A is known as a public health concern worldwide. In this study, a novel one-step real-time reverse transcription polymerase chain reaction (rtRT-PCR) assay was designed and optimized for the detection of influenza A viruses.

Material and methods

The primers and probe were designed based on the analysis of 90 matrix nucleotide sequence data of influenza type A subtypes from the GenBank database of the National Center for Biotechnology Information (NCBI). The influenza virus A/Tehran/5652/2010 (H1N1 pdm09) was used as a reference. The rtRT-PCR assay was optimized, compared with that of the World Health Organization (WHO), and its analytical sensitivity, specificity and reproducibility were evaluated. In total, 64 nasopharyngeal swabs from patients with i

SUBMITTER: Behzadi MA 

PROVIDER: S-EPMC5108395 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature