TIR-Domain-Containing Adapter-Inducing Interferon-β (TRIF) Regulates CXCR5+ T helper Cells in the Intestine.

ABSTRACT:

Objective

Establishing an effective humoral immunity is an important host defense mechanism in intestinal mucosa. T follicular helper (Tfh) cells are a spectrum of CXCR5 expressing T helper cells that induce antigen-specific B cell differentiation. Because the differentiation of T helper cells is largely regulated by innate immunity, we addressed whether TRIF signaling regulates Tfh cell differentiation and its ability to trigger humoral immune responses in the intestine.

Method

CD4⁺CXCR5⁺ T cells, B cells, and plasma cells in the Peyer's patches (PPs) of WT and TRIF-deficient (Trif^LPS2) mice were analyzed by flow cytometry at the baseline, 9 days post primary infection, and 7 days post-secondary infection with Y. enterocolitica. Y.

SUBMITTER: Kanagavelu S 

PROVIDER: S-EPMC5108586 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature