Irreversible electroporation inhibits pro-cancer inflammatory signaling in triple negative breast cancer cells.

ABSTRACT: Low-level electric fields have been demonstrated to induce spatial re-distribution of cell membrane receptors when applied for minutes or hours. However, there is limited literature on the influence on cell signaling with short transient high-amplitude pulses typically used in irreversible electroporation (IRE) for cancer treatment. Moreover, literature on signaling pertaining to immune cell trafficking after IRE is conflicting. We hypothesized that pulse parameters (field strength and exposure time) influence cell signaling and subsequently impact immune-cell trafficking. This hypothesis was tested in-vitro on triple negative breast cancer cells treated with IRE, where the effects of pulse parameters on key cell signaling factors were investigated. Importantly, real time PCR mRNA measurem

SUBMITTER: Goswami I 

PROVIDER: S-EPMC5108677 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature