T Cell Immune Deficiency in <i>zap70</i> Mutant Zebrafish.

ABSTRACT: ZAP70 [zeta-chain (TCR)-associated protein kinase, 70-kDa], is required for T cell activation. ZAP70 deficiencies in humans and null mutations in mice lead to severe combined immune deficiency. Here, we describe a zap70 loss-of-function mutation in zebrafish (zap70 ^y442 ) that was created using transcription activator-like effector nucleases (TALENs). In contrast to what has been reported for morphant zebrafish, zap70 ^y442 homozygous mutant zebrafish displayed normal development of blood and lymphatic vasculature. Hematopoietic cell development was also largely unaffected in mutant larvae. However, mutant fish had reduced lck:GFP ⁺ thymic T cells by 5 days postfertilization

SUBMITTER: Moore JC 

PROVIDER: S-EPMC5108876 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature