High-dimensional single-cell analysis reveals the immune signature of narcolepsy.

ABSTRACT: Narcolepsy type 1 is a devastating neurological sleep disorder resulting from the destruction of orexin-producing neurons in the central nervous system (CNS). Despite its striking association with the HLA-DQB1*06:02 allele, the autoimmune etiology of narcolepsy has remained largely hypothetical. Here, we compared peripheral mononucleated cells from narcolepsy patients with HLA-DQB1*06:02-matched healthy controls using high-dimensional mass cytometry in combination with algorithm-guided data analysis. Narcolepsy patients displayed multifaceted immune activation in CD4⁺ and CD8⁺ T cells dominated by elevated levels of B cell-supporting cytokines. Additionally, T cells from narcolepsy patients showed increased production of the proinflammatory cytokines IL-2 and TNF. Alt

SUBMITTER: Hartmann FJ 

PROVIDER: S-EPMC5110028 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature