Proteomic Analysis of Embryonic and Young Human Vitreous.

ABSTRACT:

Purpose

The proteomic profile of vitreous from second-trimester human embryos and young adults was characterized using mass spectrometry and analyzed for changes in protein levels that may relate to structural changes occurring during this time. This vitreous proteome was compared to previous reports to confirm proteins already identified and reveal novel ones.

Methods

Vitreous from 17 human embryos aged 14 to 20 weeks gestation (WG) and from a 12-, a 14-, a 15-, and a 28-year-old was individually analyzed using tandem mass spectrometry-based proteomics. Peptide spectral count associations with embryonic age were assessed using a general linear model of fold changes and Spearman's rank correlation. Differences between embryonic and young adult vitreous proteomes were also co

SUBMITTER: Yee KM 

PROVIDER: S-EPMC5110202 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature