Prevention of antibiotic-associated metabolic syndrome in mice by intestinal alkaline phosphatase.

ABSTRACT:

Aims

To examine whether co-administration of intestinal alkaline phosphatase (IAP) with antibiotics early in life may have a preventive role against metabolic syndrome (MetS) in mice.

Methods

A total of 50 mice were allocated to four treatment groups after weaning. Mice were treated with azithromycin (AZT) ± IAP, or with no AZT ± IAP, for three intermittent 7-day cycles. After the last treatment course, the mice were administered a regular chow diet for 5 weeks and subsequently a high-fat diet for 5 weeks. Body weight, food intake, water intake, serum lipids, glucose levels and liver lipids were compared. 16S rRNA gene pyrosequencing was used to determine the differences in microbiome composition.

Results

Exposure to AZT early in life rendered mice susceptible to MetS

SUBMITTER: Economopoulos KP 

PROVIDER: S-EPMC5110215 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature