Generating iPSC-Derived Choroidal Endothelial Cells to Study Age-Related Macular Degeneration.

ABSTRACT:

Purpose

Age-related macular degeneration (AMD), the most common cause of incurable blindness in the western world, is characterized by the dysfunction and eventual death of choroidal endothelial (CECs), RPE, and photoreceptor cells. Stem cell-based treatment strategies designed to replace photoreceptor and RPE cells currently are a major scientific focus. However, the success of these approaches likely also will require replacement of the underlying, supportive choroidal vasculature. The purpose of this study was to generate stem cell-derived CECs to develop efficient differentiation and transplantation protocols.

Methods

Dermal fibroblasts from the Tie2-GFP mouse were isolated and reprogrammed into two independent induced pluripotent stem cell (iPSC) lines via viral transdu

SUBMITTER: Songstad AE 

PROVIDER: S-EPMC5110238 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature