Mutations in VP1 and 3A proteins improve binding and replication of rhinovirus C15 in HeLa-E8 cells.

ABSTRACT: Viruses in the rhinovirus C species (RV-C) can cause severe respiratory illnesses in children including pneumonia and asthma exacerbations. A transduced cell line (HeLa-E8) stably expressing the CDHR3-Y₅₂₉ receptor variant, supports propagation of RV-C after infection. C15 clinical or recombinant isolates replicate in HeLa-E8, however progeny yields are lower than those of related strains of RV-A and RV-B. Serial passaging of C15 in HeLa-E8 resulted in stronger cytopathic effects and increased (≥10-fold) virus binding to cells and progeny yields. The adaptation was acquired by two mutations which increased binding (VP1 T₁₂₅K) and replication (3A E₄₁K), respectively. A similar 3A mutation engineered into C2 and C41 cDNAs also improved viral replication (2-8

SUBMITTER: Bochkov YA 

PROVIDER: S-EPMC5110265 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature