EstDZ3: A New Esterolytic Enzyme Exhibiting Remarkable Thermostability.

ABSTRACT: Lipolytic enzymes that retain high levels of catalytic activity when exposed to a variety of denaturing conditions are of high importance for a number of biotechnological applications. In this study, we aimed to identify new lipolytic enzymes, which are highly resistant to prolonged exposure to elevated temperatures. To achieve this, we searched for genes encoding for such proteins in the genomes of a microbial consortium residing in a hot spring located in China. After performing functional genomic screening on a bacterium of the genus Dictyoglomus, which was isolated from this hot spring following in situ enrichment, we identified a new esterolytic enzyme, termed EstDZ3. Detailed biochemical characterization of the recombinant enzyme, revealed that it constitutes a slightly

SUBMITTER: Zarafeta D 

PROVIDER: S-EPMC5110521 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature