Community-Metabolome Correlations of Gut Microbiota from Child-Turcotte-Pugh of A and B Patients.

ABSTRACT: The gut flora are widely involved in the cometabolism with the host and have evident effects on the metabolic phenotype of host. This study performed a metabolome analysis of the intestinal microbiota specific for liver cirrhosis. The study population included patients with Child-Turcotte-Pugh score of A (AP, n = 5) and B (BP, n = 5), and control subjects (NM, n = 3). Metagenomic DNA from fecal microbiota was extracted followed by metagenomic sequencing through Illumina MiSeq high throughput sequencing of 16S rRNA regions. The detection of metabolites from fecal samples was performed using high-performance liquid phase chromatography and gas chromatography coupled with tandem mass spectrometry. Intestinal microbiota community and metabolite analysis both showed separat

SUBMITTER: Wei X 

PROVIDER: S-EPMC5110571 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature