Investigating the effect of AS03 adjuvant on the plasma cell repertoire following pH1N1 influenza vaccination.

ABSTRACT: Influenza pandemics require rapid deployment of effective vaccines for control. Adjuvants such as AS03 improve vaccine immunogenicity, but this mechanism is poorly understood. We used high-throughput B cell receptor sequencing of plasma cells produced following AS03-adjuvanted and non-adjuvanted 2009 pandemic H1N1 vaccination, as well as pre-pandemic seasonal influenza vaccination to elucidate the effect of the adjuvant on the humoral immune response. By analyzing mutation levels, it was possible to distinguish sequences from cells that were recently activated from naïve B cells from those that were activated by memory recall. We show that the adjuvant functions through two mechanisms. First, the adjuvant stimulates increased activation of naïve B cells, thus reducing immune interference w

SUBMITTER: Galson JD 

PROVIDER: S-EPMC5110968 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature