The PAS Domain-Containing Protein HeuR Regulates Heme Uptake in Campylobacter jejuni.

ABSTRACT: Campylobacter jejuni is a leading cause of bacterially derived gastroenteritis. A previous mutant screen demonstrated that the heme uptake system (Chu) is required for full colonization of the chicken gastrointestinal tract. Subsequent work identified a PAS domain-containing regulator, termed HeuR, as being required for chicken colonization. Here we confirm that both the heme uptake system and HeuR are required for full chicken gastrointestinal tract colonization, with the heuR mutant being particularly affected during competition with wild-type C. jejuni Transcriptomic analysis identified the chu genes-and those encoding other iron uptake systems-as regulatory targets of HeuR. Purified HeuR bound the chuZA promoter region in electrophoretic mobility shift assays. Consistent with a role fo

SUBMITTER: Johnson JG 

PROVIDER: S-EPMC5111405 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature