Efficient Culture of Human Naive and Memory B Cells for Use as APCs.

ABSTRACT: The ability to culture and expand B cells in vitro has become a useful tool for studying human immunity. A limitation of current methods for human B cell culture is the capacity to support mature B cell proliferation. We developed a culture method to support the efficient activation and proliferation of naive and memory human B cells. This culture supports extensive B cell proliferation, with ∼10³-fold increases following 8 d in culture and 10⁶-fold increases when cultures are split and cultured for 8 more days. In culture, a significant fraction of naive B cells undergo isotype switching and differentiate into plasmacytes. Culture-derived (CD) B cells are readily cryopreserved and, when recovered, retain their ability to proliferate and differentiate. Significantly,

SUBMITTER: Su KY 

PROVIDER: S-EPMC5111638 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature